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Product Info Summary
| SKU: | RP1049 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-MBD2 Antibody
SKU/Catalog Number
RP1049
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MBD2 Antibody catalog # RP1049. Tested in WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MBD2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1049)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human MBD2 recombinant protein (Position: W159-A411). Human MBD2 shares 98% amino acid (aa) sequence identity with mouse MBD2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
RP1049 is reactive to MBD2 in Human
Applications
RP1049 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
47 kDa
Calculated molecular weight
43.255kDa
Background of MBD2
Methyl-CpG-binding domain protein 2 is a protein that in humans is encoded by the MBD2 gene. It is mapped to 18q21.2. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG-binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
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Anti-MBD2 antibody, RP1049, Western blotting
All lanes: Anti MBD2 (RP1049) at 0.5ug/m
lWB: Recombinant Human MBD2 Protein 0.5ng
Predicted bind size: 40KD
Observed bind size: 40KD
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Anti-MBD2 antibody, RP1049, Western blotting
All lanes: Anti MBD2 (RP1049) at 0.5ug/ml
Lane 1: SGC Whole Cell Lysate at 40ug
Lane 2: HELA Whole Cell Lysate at 40ug
Lane 3: JURKAT Whole Cell Lysate at 40ug
Lane 4: K562 Whole Cell Lysate at 40ug
Predicted bind size: 47KD
Observed bind size: 47KD
Protein Target Info & Infographic
Gene/Protein Information For MBD2 (Source: Uniprot.org, NCBI)
Gene Name
MBD2
Full Name
Methyl-CpG-binding domain protein 2
Weight
43.255kDa
Alternative Names
demethylase; DKFZp586O0821; DMTase; methyl-CpG binding domain protein 2; methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41 MBD2 DMTase, NY-CO-41 methyl-CpG binding domain protein 2 methyl-CpG-binding domain protein 2|demethylase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MBD2, check out the MBD2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MBD2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MBD2 Antibody (RP1049)
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No publications found for RP1049
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Customer Reviews
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Customer Q&As
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1 Customer Q&As for Anti-MBD2 Antibody
Question
We are currently using anti-MBD2 antibody RP1049 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2019-07-17
Answer
The anti-MBD2 antibody (RP1049) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-17
